Us versus Them: The Debates on the Legislation of Same-Sex Marriage (1994 - 2015) in Taiwan.

Taiwan, the first country in Asia to provide same-sex couples a legal right to marriage, has witnessed furious debate on both sides. To understand this debate, we combined content analysis and the Linguistic Inquiry and Word Count (LIWC) method to capture themes and states of mind reflected in the news articles. Arguments based in human rights and reflecting heterosexual preferences which were observed elsewhere also dominated the discourses in Taiwan. First-person pronouns, positive and negative emotions, and differentiation were found to be associated with rights discourses and, in turn, support for same-sex marriage. The themes, the state of mind of the involved parties, and same-sex marriage legalization are further discussed.

Social Monitoring Matters for Deterring Social Deviance in Stable but Not Mobile Socio-Ecological Contexts.

Previous research suggests that reputational concerns can incentivize cooperation and deter socially deviant behavior. The current research showed that social monitoring of information that has the potential to damage one's reputation has differential effects on deviant behavior in social-ecological environments that vary in level of mobility. Study 1 showed that residentially stable cities that employed more journalists-who can be regarded as social monitoring agents in a community-tended to have lower rates of violent crime than residentially stable cities that employed fewer journalists; by contrast, in residentially mobile cities, violent crime rates did not vary as a function of the number of journalists employed. In Study 2, we found that individual differences in perceptions of relational mobility moderated the effects of social monitoring on cheating in a die-under-cup game. Specifically, social monitoring cues reduced the likelihood of cheating but only among participants who perceived their immediate social environment to be low in relational mobility. The same results were replicated in Study 3, an experiment in which participants' perception of relational mobility was manipulated before completing an online maze game that allowed them to earn extra cash. In the low mobility condition, the percentage of participants who continued working on the mazes after reaching the time limit decreased as a function of social monitoring; however, this pattern was not observed in the high mobility condition. Together, our findings suggest that socioecological context matters for understanding effective mechanisms of social control.

A Strategy for Generating a Broad-Spectrum Monoclonal Antibody and Soluble Single-Chain Variable Fragments against Plant Potyviruses.

Potyviruses are major pathogens that often cause mixed infection in calla lilies. To reduce the time and cost of virus indexing, a detection method for the simultaneous targeting of multiple potyviruses was developed by generating a broad-spectrum monoclonal antibody (MAb) for detecting the greatest possible number of potyviruses. The conserved 121-amino-acid core regions of the capsid proteins of Dasheen mosaic potyvirus (DsMV), Konjak mosaic potyvirus (KoMV), and Zantedeschia mild mosaic potyvirus (ZaMMV) were sequentially concatenated and expressed as a recombinant protein for immunization. After hybridoma cell fusion and selection, one stable cell line that secreted a group-specific antibody, named C4 MAb, was selected. In the reaction spectrum test, the C4 MAb detected at least 14 potyviruses by indirect enzyme-linked immunosorbent assay (I-ELISA) and Western blot analysis. Furthermore, the variable regions of the heavy (VH) and light (VL) chains of the C4 MAb were separately cloned and constructed as single-chain variable fragments (scFvs) for expression in Escherichia coli. Moreover, the pectate lyase E (PelE) signal peptide of Erwinia chrysanthemi S3-1 was added to promote the secretion of C4 scFvs into the medium. According to Western blot analysis and I-ELISA, the soluble C4 scFv (VL-VH) fragment showed a binding specificity similar to that of the C4 MAb. Our results demonstrate that a recombinant protein derived from fusion of the conserved regions of viral proteins has the potential to produce a broad-spectrum MAb against a large group of viruses and that the PelE signal peptide can improve the secretion of scFvs in E. coli.